ASTM Laboratory Test Methods for Fungi, Bacteria, and Algae

Material Fungal, Bacterial and Algal Test Methods

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ASTM G21 - Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi. The ASTM G21 test is for testing antifungal and fungicide treated products.

ASTM D3273 - Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber. The ASTM D3273 is for testing antifungal and fungicide treated products, and unlike the G21, the D3273 has a durability component as part of the method..

ASTM D5590 - Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay. The ASTM D5590 is for testing antifungal and fungicide treated products in a plate assay.

ASTM D5589 - Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Algal Defacement.

ASTM G29 - Standard Practice for Determining Algal Resistance of Plastic Films. The ASTM G29 algae test method is for testing algae and algicide formulations or treated products.

ASTM E2149 - Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. The ASTM E2149 is used in testing textiles that have been treated with antibacterial or biocides. Quat-Silane antimicrobials are commonly testing using this method.

ASTM D2574 - Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms. The ASTM D2574 is a preservation test for testing biocides in-can preservative performance. The D2574 can be used to test resistance of a product to fungi, algae or bacteria.

ASTM E2563 - Standard Test Method for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method. The ASTM E2563 is a microbial test for bacteria, particularly slow growing Mycobacteria sp.

ASTM E2564 - Standard Test Method for Enumeration of Mycobacteria in Metalworking Fluids by Direct Microscopic Counting (DMC) Method. ASTM E2564 is a microbial test for bacteria, particularly slow growing Mycobacteria sp.

ASTM E2315 - Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill Procedure. The ASTM E2315 is typically used to test disinfectants against bacteria and yeast fungi.

ASTM D5588 - Standard Test Method for Determination of the Microbial Condition of Paint, Paint Raw Materials, and Plant Areas. ASTM D5588 is a test methods commonly used in preservation of water based formulation against bacteria and fungi.

ASTM E2275 - Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance. ASTM E2275 is commonly used in testing metal working fluid MWF preservation against bacteria and fungi, including Mycobacteria sp.

ASTM E686 - Evaluates the utility and effectiveness of antibacterial and antifungal agents at use concentrations in controlling the microorganisms responsible for the deterioration of metal-working fluids. This method is currently withdrawn in favor of ASTM E2275. ASTM E686 is the former standard for metal working fluid MWF testing.

ASTM E1259 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C. ASTM E1259 is one of few methods for determining the preservation of fuel and biofuel products.

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Antimicrobial Testing

Fungi are ubiquitous, any given environment will have the potential for fungal contamination. Formulation additives such as antimicrobials and hydrophobic auxiliaries can work synergistically to eliminate fungal growth.

In building and construction applications, address the structural and environmental issues first and then assess the need for an antifungal product.

There are several test agencies and suppliers for antimicrobials and biodegradable products. Agencies such as EMSL, Microbiotest, Biosan, and Bureau Veritas are well known for their testing of ASTM, ISO, EPA and FDA antimicrobial and biodegradability test methods. Suppliers such as Dow Chemical, Arch, Milliken, Buckman, ISP, and other antimicrobial additive companies supply additives for construction material preservation, bacterial, fungal and algal preservation and remediation. Situ Biosciences has worked with global suppliers and customers to make their products the best performing, most cost effective and with the least environmental impact for biodegradability and antimicrobial properties.

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Preservation, Antimicrobial, and Biodegradable Products

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