Upcoming Expo &
Technical Conference
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OECD 218/219 – Sediment-Water Toxicity Test

OECD 218 and 219 are Sediment-Water Chironomid Toxicity tests using Spiked Sediment (218) or Spiked Water (219).sediment dwellers come into direct contact with chemicals found in sediment and also serve as an important food source for other predatory animals, allowing bioaccumulation to occur from persistent chemicals.

OECD 218 and 219 are designed to assess the effects of prolonged exposure of chemicals to the sediment-dwelling larvae of the freshwater dipteran Chironomus sp. It is based on existing toxicity test protocols for􏰀 Chironomus riparuis and Chironomus tentans.

The chronic Chironomus studies are performed following two different exposure scenarios:

  • spiking of the water column to simulate a pesticide spray drift (OECD 219*)
  • spiking of the sediment to address risks from exposure to contaminated sediment, particularly if there is an accumulation of the compound in the sediment over time (OECD 218*)

Standard testing for both OECD 218 and 219 runs for approximately 20-28 days for Chironomus riparuis and 28-65 for Chironomus tentans.  For these standard test methods, the measured endpoints are the total number of adults emerged and the time to emergence.

It is  important to have data on the test substance’s water solubility (OECD 105)* and the vapour pressure.  It can also be beneficial to have data on the different performance and physical and chemical properties of the material including structural formula, purity of the substance, stability in water and light, the Partition Coefficient (OECD 117)*, Dissociation Constants in water (OECD 112)*, and results of a test for ready biodegradability.

Many toxicology tests are used to meet regulatory requirements for different product applications; for any testing performed to meet regulatory or third party labeling requirements, it is strongly recommended to review requirements prior to testing with the appropriate agency to determine which tests should be performed for label claims or regulatory acceptance.

*OECD 218: Sediment-Water Chrionomid Toxicity Test Using Spiked Sediment

*OECD 219: Sediment-Water Chrionomid Toxicity Test Using Spiked Water

For more information on Toxicology Testing, contact our Environmental Testing laboratory at 847-483-9950 or info@situtest.com.

Situ Biosciences is Exhibiting in the Advanced Textiles Testing Demo Zone!

@ IFAI Expo 2017

September 26-29, 2017
New Orleans, LA

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Let us know if you’d like to have a meeting at the conference!

847-483-9950 – info@situtest.com