OECD 307: Aerobic and Anaerobic Transformation in Soil
The OECD 307 measures the biodegradation of a substance by transformation in soil.
OECD 307 is a biodegradation method for chemicals in a soil environment. Chemicals can enter these types of environments by direct application, run-off, drainage, waste disposal, etc. Although this method is performed in an artificial laboratory system, the results allow an estimation of the test substance’s rate of transformation and the rate of formation and decline of transformation products in field conditions. The OECD 307 provides information on the way the test substance is structurally changed in the soil by chemical and microbial reactions. Such tests are required for chemicals that will be directly applied to soil or that are likely to reach the soil environment.
Soil samples are treated with the test substance, and then incubated in darkness under controlled laboratory conditions (at constant temperature, etc.). At several time points, soil samples are taken and analyzed for the test substance and any subsequent transformation products that may have formed. The duration of the method generally does not exceed 120 days.
Before performing the OECD 308, information should be available on the test substance’s solubility in water (OECD 105), solubility in organic solvents, vapor pressure , n-octanol/water partition coefficient (OECD 117), dissociation constant (pKa), and chemical structure. Other useful information includes toxicity to microorganisms and ready or inherent biodegradability.
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