OECD 309: Aerobic Mineralisation in Surface Water – Simulation Biodegradation
The OECD 309 determines the mineralization of a test substance in surface water.
The main objective of the OECD 309 is to measure the mineralization (biodegradation) of a low concentration of an organic test substance in aerobic natural surface water (fresh, brackish or marine). It is a laboratory shake flask test to determine a product’s rates of aerobic biodegradation. An optional secondary objective is to obtain information on the product’s primary degradation and formation of transformation products.
During the OECD 309, the test substance is incubated with either surface water only (“pelagic test”) or with turbid surface water with suspended solids/sediment (“suspended sediment test”). The samples are incubated in the dark at a controlled temperature under aerobic conditions and agitation. Degradation is measured at various time points. The duration of the test is generally up to 60 days.
Information on biodegradability of the test substance at higher concentrations, information on abiotic degradability, transformation products and relevant physico-chemical properties should be available prior to the test to help establish the experimental planning and interpret the results. If the “suspended sediment test” is performed, the – n-octanol/water partition coefficient [OECD 117] and adsorption coefficient [OECD 106] of the test substance should also be available before testing.
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