Tag Archives: Liquid Biodegradation

OECD 306 – Biodegradation Test – Seawater

OECD 306 is a biodegradation test that specifically shows how  a material biodegrades in seawater. OECD 306 is very similar to the OECD 301 series, however, OECD 306 requires the use of a seawater inoculum and is often used in conjunction with the OECD 301/302 Sludge Inoculum protocol.  Testing is generally run for 30 days but can last […]

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OECD 301B – Biodegradation Test – CO2 Evolution

OECD 301B is an aerobic biodegradation test that introduces a material to an inoculum in a closed environment and measures biodegradation of the material by CO2 evolution. OECD 301B uses respirometry to determine biodegradability of the material by evaluating the production of CO2 over a minimum of 28 days in a liquid environment. The OECD 301B test method can be used […]

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OECD 302B – Inherent Biodegradation

OECD 302B is an inherent aerobic biodegradation test used for determining the biodegradability of a solution typically not readily biodegradable, is known to be insoluble or does not satisfy the requirements of OECD 301. For OECD 302B, the determination of Dissolved Organic Carbon (DOC) dominantly monitors the biodegradation process; however, Chemical Oxygen Demand (COD) can also be used. Standard […]

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OECD 310 – Ready Biodegradation – CO2

OECD 310 is an aerobic biodegradation test that predominantly measures ready biodegradability by the evaluation of CO2 in sealed vessels. In the OECD 310 test method, the CO2 evolution resulting from the ultimate aerobic biodegradation of the test substance is determined by measuring the inorganic carbon (IC) produced in sealed test bottles. Standard testing for OECD 310 is […]

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MIL-PRF 87937D – Biodegradation Test – Surfactants

MIL-PRF 87937D is a performance test for electronic equipment cleaner surfactants that determines biodegradation by the Shake Flask Method. The MIL-PRF 87937D establishes the requirements for biodegradable, water dilutable, and environmentally safe cleaning compounds for use on aerospace equipment.  Biodegradability of the finished product is determined by the standard testing of 28 days by the […]

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ISO 14593 – Ultimate Aerobic Biodegradation

ISO 14593 is a solution biodegradation test that performs an evaluation of ultimate aerobic biodegradability of organic compounds in an aqueous medium. Ultimate aerobic biodegradation: Microorganisms fully consume a chemical compound or organic matter by in the presence of oxygen. Produces carbon dioxide, water and mineral salts. The ISO 14593 method analyzes inorganic carbon in sealed vessels (CO2 headspace test). It […]

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OECD 301F – Biodegradation Test – O2 Consumption

OECD 301F is a solutions aerobic biodegradation test that determines the biodegradability of a material by measuring oxygen consumption. OECD 301F is most often used for insoluble and volatile materials that are challenged by OECD 301B testing.  The purity or proportions of major components of the test material is important for calculating the Theoretical Oxygen Demand (ThOD). Similarly to other 301 test […]

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OECD 301D – Biodegradation Closed Bottle Test

OECD 301D is an aerobic biodegradation test method that can be used with poorly soluble materials and is suitable for volatile and absorbing material samples. OECD 301D is a respirometry test that predominantly measures biodegradation by dissolved oxygen and testing is a minimum of 28 days. Similar to other OECD 301 biodegradation methods, OECD 301D can test for Ready, […]

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OECD 301A – Biodegradation Test – DOC

OECD 301A is an aerobic biodegradation test used for non-volatile and soluble (100mg/L) substances. In the OECD 301A test method, the degree of biodegradation is measured by the change in Dissolved Organic Carbon (DOC) over a 28-Day period.  It is similar to the OECD 301E, but higher microbial cell densities can be used in the OECD 301A. OECD […]

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ASTM D5864 – Lubricant Biodegradation Test

ASTM D5864 is a biodegradation test method for determining aerobic aquatic biodegradation of lubricants or their components. ASTM D5864 determines the degree of aerobic aquatic biodegradation of fully formulated lubricants or their components, based on the exposure to an inoculum under laboratory conditions. Testing is designed to be applicable to all lubricants that are not volatile and are not inhibitory at the test […]

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