ASTM E1052: Efficacy of Antimicrobial Agents Against Viruses in Suspension

The ATSM E1052 test is performed to assess the efficacy of antimicrobials against suspended viruses. This is performed by utilizing eukaryotic cell cultures as host systems.

This suspension test is meant for special virucidal applications. This includes inactivation of viruses in contaminated liquid wastes as well as determination of virucidal activity for liquid chemicals or personal care products.

A general example of the test is performed as follows; the virus is exposed to the virucide for a defined period of time to reflect actual conditions of use for the product. After the appropriate exposure interval, the mixture is evaluated for viable virus in the appropriate host system. Per the ASTM E1052 method, controls for cell culture, cytotoxicity, and susceptibility to viruses are also included in the testing. This allows to determine if viruses in suspension can be inactivated by the tested product.

Additional variables such as temperature are also reflective of those expected for actual customer use. As needed, a durability assessment can be accommodated into the testing to simulate an organic soil load or a hard water environment for products expected to be applied in high/heavy use conditions

Commonly requested viral strains for the ASTM E1052 method include:

  • Influenza A virus (H1N1 and H3N2)
  • Feline calicivirus
  • Feline coronavirus
  • Human coronavirus* (229E and OC43)

*SARS-CoV-2 (COVID-19) currently not available.

Additional viral groups can be accommodated as needed.

Depending on the end use of the product, testing in accordance with Good Laboratory Practice (GLP) guidelines may be determined necessary per regulatory requirements. Our laboratory can provide both GLP or non-GLP testing.

Comparable ASTM methods designated for the assessment of antimicrobial effectiveness include ASTM E1053, and ASTM E1153.

For more information on Antimicrobial Testing email info@situtest.com or call (847) 483-9950.