OECD 308: Aerobic and Anaerobic Transformation in Aquatic Sediment Systems
The OECD 308 measures the biodegradation of a substance by aerobic and anaerobic transformation in aquatic sediment systems.
OECD 308 is a biodegradation method for chemicals in an aquatic sediment environment. Chemicals can enter these types of environments by direct application, run-off, drainage, waste disposal, etc. This method consists of aerobic and anaerobic tests to best simulate common aquatic sediment conditions. The surface layer of sediment can be either aerobic or anaerobic, whereas the deeper sediment is usually anaerobic. Other condition specifications can be made upon discussion with the lab. Although this method is performed in an artificial laboratory system, the results allow an estimation of the test substance’s rate of transformation and the rate of formation and decline of transformation products in field conditions. The OECD 308 provides information on the way the test substance is structurally changed in aquatic sediment systems by chemical and microbial reactions.
Aquatic sediment samples are set up in both aerobic and anaerobic test systems and are treated with the test substance. Test systems are then incubated in darkness under controlled laboratory conditions (at constant temperature, etc.). At several time points, sediment samples are taken and analyzed for the test substance and any subsequent transformation products that may have formed. The duration of the method generally does not exceed 100 days.
Before performing the OECD 308, information should be available on the test substance’s solubility in water (OECD 105), solubility in organic solvents, vapor pressure , n-octanol/water partition coefficient (OECD 117), adsorption coefficient (OECD 121), hydrolysis, dissociation constant (pKa), and chemical structure. Other useful information includes toxicity to microorganisms, ready and/or inherent biodegradability, and aerobic and anaerobic transformation in soil.
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