OECD 315 – Bioaccumulation in Sediment-dwelling Benthic Oligochaetes

OECD 315 is a bioaccumulation test on benthic oligochaetes (aquatic sediment-dwelling worms).

The OECD 315 method measures the residues of a test substance in earthworms after exposure to the test substance mixed with soil.  The method consists of two phases- the uptake phase and elimination phase. Bioaccumulation rate is assessed after both phases are complete. OECD 315 testing is useful for any chemical that may come into contact with soil.

Test Organisms

For OECD 315, commonly used species of benthic oligochaetes (aquatic sediment-dwelling worms) are Tubifex tubifex, Lumbriculus variegates, and Branchiura sowerbyi.

Method Summary

Worms are exposed to the test substance in the treated sediment and topped with water for 28 days (uptake phase). Worm and sediment samples are then analyzed for the test substance. At the end of the uptake phase the worms from remaining test units are transferred to untreated sediment, topped with water, and incubated for 10 days (elimination phase). Worm samples are again analyzed for the test substance.


Three parameters determine the sample’s bioaccumulation: bioaccumulation factor (BAF), uptake rate constant (ks) and elimination rate constant (ke).

For more information on OECD 315, contact the lab at 847-483-9950 or info@situtest.com

Situ Biosciences to Present at Personal Care EXPO

Come check out our booth #332!

Situ Biosciences will be at the Teamworks Expo in April to showcase our technical expertise in the personal care and cosmetic industry. Our team will go over product performance testing and formulation development, and be able to discuss a variety of services that help our customers meet their testing needs.

When: April 11, 2018

Located at the Donald E. Stephens Convention Center:
5555 N River Rd, Rosemont, IL 60018

Come visit our booth: #332

Please let us know if you would  like to set up a meeting at the conference.

Feel free to contact our laboratory at (847) 483-9950 or info@situtest.com