OECD 211: Daphnia Magna Reproduction Test

OECD 211 is a Reproduction test that determines a material’s effect on Daphnia Magna reproduction and mobilization.

OECD 211 assess the effect of chemicals on the reproductive output of Daphnia magna.  Standard testing is performed by exposing young female Daphnia (the parent animals), aged less than 24 hours at the start of the test, to the test substance added to water at a range of concentrations.

The OECD 211 standard test duration is 21 days. At the end of the test, the total number of living offspring produced is assessed.  Results of an acute toxicity test (see OECD 202*) performed with Daphnia magna may be useful in selecting an appropriate range of test concentrations in the reproduction tests.

It is important to have data on the test substance’s water solubility (OECD 105)* and the vapor pressure.  It can also be beneficial to have data on the different performance and physical and chemical properties of the material including structural formula, purity of the substance, stability in water and light, the Partition Coefficient (OECD 117)*, Dissociation Constants in water (OECD 112)*, and results of a test for ready biodegradability.

Although many applications utilize the information produced form OECD 211 testing, customers commonly request OECD 211 testing to meet a component of the OECD and EU Ecotoxicity requirements for REACH chemicals.  However, many toxicology tests are used to meet regulatory requirements for different types of product applications.

For any testing performed to meet regulatory or third party labeling requirements, it is strongly recommended to review requirements prior to testing with the appropriate agency to determine which tests should be performed for label claims or regulatory acceptance.

*OECD 202: Daphnia sp. Acute Immobilization Test

* OECD 105: Water Solubility

*OECD 112: Dissociation Constants in Water

*OECD 117: Partition Coefficient (n-octanol/water), HPLC Method

For more information on Toxicology Testing, contact our Environmental Testing laboratory at 847-483-9950 or info@situtest.com.