Protocol 01-1A: Protocol for Residual Self-Sanitizing Activity of Dried Chemical Residues on Hard, Non-Porous Surfaces

This EPA disinfection test is designed to asses the residual sanitizing efficacy of antimicrobial products intended for hard, inanimate, non-porous, and non-food contact surfaces such as glass, stainless steel, or plastics. The method consists of a “wear” and reinoculation procedure as well as a sanitizer test. It is a quantitative approach which allows for a pass/fail evaluation of the test product.

The “wear” and reinoculation of the test and control surfaces is performed over a 24-hour time interval. Each abrasion cycle is performed after at least 30 minutes of dry time following previous reinoculation, and each reinoculation is performed after a 15-minute dry period following each abrasion. Once this is completed, the sanitizer test is performed. Each treated and control sample is inoculated with the test culture. Per the method standard, the samples are evaluated after a 5-minute exposure period and then neutralized. For a product to be considered a sanitizer, it must achieve at least 99.9% reduction observed on the tested surface.

Standard organisms tested against in the Protocol 01-1A method include:

• Staphylococcus aureus
• Klebsiella pneumoniae
• Klebsiella aerogenes (also known as Enterobacter aerogenes)

Additional microorganisms can be included a needed.

Regulatory requirements may include testing in accordance with the Good Laboratory Practice (GLP) guidelines. Our laboratory can provide both GLP or non-GLP testing. We highly recommend contacting the appropriate agencies to confirm the necessary testing criteria.

For more information on Antimicrobial Testing email info@situtest.com or call (847) 483-9950.