EPA – OPPTS 850.1035: Mysid Acute Toxicity Test

This EPA OPPTS 850.1035 method is an acute toxicity test designed to evaluate the impact of chemical substances and mixtures present in the aquatic environment. 

The purpose of this method is to determine the acute toxicity of a material on saltwater mysids in a static, static-renewal, or flow-through system, which allows to assess the hazards and risks of the material in aqueous conditions.

In this test, young mysids are exposed to the test substance for a period of 96 hours. During this time, organism survival as well as other toxic effects are observed, and a concentration-response curve, expressed as the 96-hour lethal concentration, is established.

OPPTS 850.1035: Mysid Acute Toxicity evaluation consist of range-finding, as well as definitive and limit testing. Once the appropriate test solution concentrations are determined during the range-finding step, definitive testing is performed to establish the 96-hour concentration-response curve. A limit test can then be performed to demonstrate the concentration-response curve is above a certain limit concentration.

Low water soluble materials may require a Water Accommodated Fraction (WAF) analysis prior to toxicology testing. WAF is an adaptive pre-analysis that can address the solubility issue for non-aqueous samples by preparing water-accommodated fractions of the material.

It is strongly recommended to review necessary requirements prior to testing with the appropriate regulatory agency to determine which tests should be performed for label claims or regulatory acceptance.

Testing performed to meet regulatory or third party branding requirements, may require testing in accordance with GLP guidelines. At Situ Biosciences, our test laboratory can provide GLP as well as non-GLP testing.

For more information on Toxicology Testing, contact our test laboratory at info@situtest.com or call 847-483-9950.