Tag Archives: Liquid Biodegradation

OECD 302B is an inherent aerobic biodegradation test used for determining the biodegradability of a solution typically not readily biodegradable, is known to be insoluble or does not satisfy the requirements of OECD 301. For OECD 302B, the determination of Dissolved Organic Carbon (DOC) dominantly monitors the biodegradation process; however, Chemical Oxygen Demand (COD) can also be used. Standard […]

OECD 302B – Inherent Biodegradation

OECD 310 is an aerobic biodegradation test that predominantly measures ready biodegradability by the evaluation of CO2 in sealed vessels. In the OECD 310 test method, the CO2 evolution resulting from the ultimate aerobic biodegradation of the test substance is determined by measuring the inorganic carbon (IC) produced in sealed test bottles. Standard testing for OECD 310 is […]

OECD 310 – Ready Biodegradation – CO2

ISO 14593 is a solution biodegradation test that performs an evaluation of ultimate aerobic biodegradability of organic compounds in an aqueous medium. Ultimate aerobic biodegradation: Microorganisms fully consume a chemical compound or organic matter by in the presence of oxygen. Produces carbon dioxide, water and mineral salts. The ISO 14593 method analyzes inorganic carbon in sealed vessels (CO2 headspace test). It […]

ISO 14593 – Ultimate Aerobic Biodegradation

ISO 11734 is a solution anaerobic biodegradation test that evaluates ultimate anaerobic biodegradability of organic compounds in digested sludge. Measurement for analysis for ISO 11734 is done by biogas production. The ISO 11734 test method applies to organic compounds with a known carbon content and are: Soluble in water, or Poorly soluble in water, provided exact dosing […]

ISO 11734 – Anaerobic Biodegradation

At Situ Biosciences, we are committed to providing accurate and reliable biodegradability testing services to businesses across various industries. Biodegradation product testing, when intended for product claims related to biodegradability, should incorporate several factors of the product’s actual performance.  For example, products successfully tested to ‘Ready Biodegradability’ standards using the OECD 301B biodegradation test method can make […]

Biodegradability Testing

Organization for Economic Co-operation & Development (OECD) OECD is recognized globally and is most commonly chosen by our customers for solution-based testing for lubricants, oils, detergents, personal care products, solvents and materials that dissolve in everyday use such as tablets and powders. In addition to promoting global economic cooperation and development, the OECD develops and […]


Solutions and Liquid Testing Overview Used for determination of Ready, Ultimate and/or Inherent Biodegradability OECD 301 Biodegradation methods typically run for 28 days; OECD 302 Biodegradation Methods are intended for longer term testing; with more complicated solutions. Many other harmonized methods and variants of the OECD method exist.  The differences between these method are due […]

Biodegradation: DOC, CO2, O2 Measurements & Methods

Although great effort is put into prevention, its standard practice to presume that some lubricant will enter the environment. The ability to biodegrade and maintain low toxicity to organisms are key considerations in product development; especially for sensitive environments than could see a significant impact from the introduction of potentially hazardous liquids. For customers in the energy […]

Lubricant Biodegradation & Toxicology Testing