The ASTM D5660 test method is the legacy standard test method for assessing the microbial detoxification of chemically contaminated water and soil using a toxicity test with a luminescent marine bacterium, Photobacterium phosphoreum. Testing provides valuable information on how a chemical may contaminate water or soil while in use and after disposal.  Sensitive environments can be greatly affected by even […]

ASTM D5660 – Phototoxicity Testing

ASTM D5864 is a biodegradation test method for determining aerobic aquatic biodegradation of lubricants or their components. ASTM D5864 determines the degree of aerobic aquatic biodegradation of fully formulated lubricants or their components, based on the exposure to an inoculum under laboratory conditions. Testing is designed to be applicable to all lubricants that are not volatile and are not inhibitory at the test […]

ASTM D5864 – Lubricant Biodegradation Test

ASTM D6081 is the standard test for aquatic toxicity testing of lubricants and can test for acute or chronic toxicity. Chronic Analysis typically consists of exposing organisms to a level of the material and then measuring the bioaccumulation of that material in the organisms over a specified exposure time. Acute Analysis typically is done by establishing […]

ASTM D6081 – Algal Toxicity Testing

OECD 105 determines the water solubility of a substance. OECD 105 determines the water solubility of essentially pure substances which are stable in water and not volatile. It is important to note that the presence of impurities can considerably affect the water solubility of a substance. It is recommended to have preliminary information on the test substance’s structural […]

OECD 105 – Water Solubility

OECD 111 assesses abiotic hydrolytic transformations of chemicals in aquatic systems at pH values normally found in the environment (pH 4 – 9). OECD 111 is an important evaluation for determining how a chemical will react in an aqueous environment; hydrolysis itself is an important reaction controlling degradation.  Abiotic hydrolysis is likely one of the most common […]

OECD 111 – Hydrolysis as a Function of pH

OECD 113 is a preliminary assessment to evaluate the stability of a substance with respect to heat and air. OECD 113 is commonly used to determine stability and is used as guidance in the performance of other tests. The determination of storage stability is applicable to homogeneous solid and liquid substances and to mixtures of these. Two principle test methods […]

OECD 113 – Thermal Stability and Stability in Air

OECD 121 estimates the adsorption coefficient on soil and sewage sludge using high-performance liquid chromatography (HPLC).   The adsorption coefficient (Koc) determines the tendency of a chemical to bind to soils or sewage sludge. The binding capacity of a chemical on organic matter of soil and sewage sludge allows comparisons to be made between different chemicals through their adsorption behavior. The […]

OECD 121 – Adsorption Coefficient using HPLC

OECD 201 is a standard freshwater algae and cyanobacteria Growth inhibition test commonly used for aquatic toxicology testing. OECD 201 determines the effects a substance has on the growth of freshwater micro-algae or cyanobacteria through an acute aquatic plant growth toxicity test.  OECD 201 exposes exponentially growing organisms to the test substance over a 72 hour test […]

OECD 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test

OECD 202 is an acute immobilization toxicology test that determines the concentrations of a material that immobilizes Daphnia species. Standard testing for OECD 202 is performed by exposing young daphnids to the test substance at a range of concentrations for a period of 48 hours. Immobilization is recorded at 24 hours and 48 hours and compared with […]

OECD 202: Daphnia Sp., Immobilization Test

OECD 203 is an acute toxicity test that evaluates the toxicity of a test substance to different fish species. Standard testing for OECD 203 exposes fish to a test substance for a period of 96 hours. Mortalities are recorded at 24, 48, 72, and 96 hours, and the concentrations that kill 50% of the fish are determined when possible. At least five […]

OECD 203: Fish, Acute Toxicity Test

OECD 210 is a toxicity test against fish in their early life stage. OECD 210 exposes fish in their early life stage to a range of concentrations of the test chemical dissolved in water. Tests done on early-life stages of fish are intended to define the lethal and sub-lethal effects of chemicals on the stages and […]

OECD 210: Fish, Early-life Stage Toxicity Test

OECD 211 is a Reproduction test that determines a material’s effect on Daphnia Magna reproduction and mobilization. OECD 211 assess the effect of chemicals on the reproductive output of Daphnia magna.  Standard testing is performed by exposing young female Daphnia (the parent animals), aged less than 24 hours at the start of the test, to the […]

OECD 211: Daphnia Magna Reproduction Test