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The ASTM D5660 test method is the legacy standard test method for assessing the microbial detoxification of chemically contaminated water and soil using a toxicity test with a luminescent marine bacterium, Photobacterium phosphoreum. Testing provides valuable information on how a chemical may contaminate water or soil while in use and after disposal.  Sensitive environments can be greatly affected by even […]

ASTM D5660 – Phototoxicity Testing

ISO 11734 is a solution anaerobic biodegradation test that evaluates ultimate anaerobic biodegradability of organic compounds in digested sludge. Measurement for analysis for ISO 11734 is done by biogas production. The ISO 11734 test method applies to organic compounds with a known carbon content and are: Soluble in water, or Poorly soluble in water, provided exact dosing […]

ISO 11734 – Anaerobic Biodegradation

ISO 14593 is a solution biodegradation test that performs an evaluation of ultimate aerobic biodegradability of organic compounds in an aqueous medium. Ultimate aerobic biodegradation: Microorganisms fully consume a chemical compound or organic matter by in the presence of oxygen. Produces carbon dioxide, water and mineral salts. The ISO 14593 method analyzes inorganic carbon in sealed vessels (CO2 headspace test). It […]

ISO 14593 – Ultimate Aerobic Biodegradation

MIL-PRF 87937D is a performance test for electronic equipment cleaner surfactants that determines biodegradation by the Shake Flask Method. The MIL-PRF 87937D establishes the requirements for biodegradable, water-dilutable, and environmentally safe cleaning compounds for use on aerospace equipment.  Biodegradability of the finished product is determined by the standard testing of 28 days by the Shake […]

MIL-PRF 87937D – Biodegradation Test – Surfactants

OECD 105 determines the water solubility of a substance. OECD 105 determines the water solubility of essentially pure substances which are stable in water and not volatile. It is important to note that the presence of impurities can considerably affect the water solubility of a substance. It is recommended to have preliminary information on the test substance’s structural […]

OECD 105 – Water Solubility

OECD 112 assesses the dissociation of a chemical in water. The OECD 112 analysis is important in assessing a materials impact on the environment.  In part, because the dissociation constant governs the form of the substance, which then determines its behavior and transport. Dissociation is the reversible splitting into two or more chemical species.  Typically, the dissociation constant measures the […]

OECD 112 – Dissociation Constants in Water

OECD 113 is a preliminary assessment to evaluate the stability of a substance with respect to heat and air. OECD 113 is commonly used to determine stability and is used as guidance in the performance of other tests. The determination of storage stability is applicable to homogeneous solid and liquid substances and to mixtures of these. Two principle test methods […]

OECD 113 – Thermal Stability and Stability in Air

OECD 121 estimates the adsorption coefficient on soil and sewage sludge using high-performance liquid chromatography (HPLC).   The adsorption coefficient (Koc) determines the tendency of a chemical to bind to soils or sewage sludge. The binding capacity of a chemical on organic matter of soil and sewage sludge allows comparisons to be made between different chemicals through their adsorption behavior. The […]

OECD 121 – Adsorption Coefficient using HPLC

OECD 201 is a standard freshwater algae and cyanobacteria Growth inhibition test commonly used for aquatic toxicology testing. OECD 201 determines the effects a substance has on the growth of freshwater micro-algae or cyanobacteria through an acute aquatic plant growth toxicity test.  OECD 201 exposes exponentially growing organisms to the test substance over a 72 hour test […]

OECD 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test

OECD 202 is an acute immobilization toxicology test that determines the concentrations of a material that immobilizes Daphnia species. Standard testing for OECD 202 is performed by exposing young daphnids to the test substance at a range of concentrations for a period of 48 hours. Immobilization is recorded at 24 hours and 48 hours and compared with […]

OECD 202: Daphnia Sp., Immobilization Test

OECD 203 is an acute toxicity test that evaluates the toxicity of a test substance to different fish species. Standard testing for OECD 203 exposes fish to a test substance for a period of 96 hours. Mortalities are recorded at 24, 48, 72, and 96 hours, and the concentrations that kill 50% of the fish are determined when possible. At least five […]

OECD 203: Fish, Acute Toxicity Test

OECD 210 is a toxicity test against fish in their early life stage. OECD 210 exposes fish in their early life stage to a range of concentrations of the test chemical dissolved in water. Tests done on early-life stages of fish are intended to define the lethal and sub-lethal effects of chemicals on the stages and […]

OECD 210: Fish, Early-life Stage Toxicity Test